True monolayer cell culture in a confined 3D microenvironment enables lineage informatics
نویسندگان
چکیده
منابع مشابه
Synthetic sandwich culture of 3D hepatocyte monolayer.
The sandwich culture of hepatocytes, between double layers of extra-cellular matrix (ECM), is a well-established in vitro model for re-establishing hepatic polarity and maintaining differentiated functions. Applications of the ECM-based sandwich culture are limited by the mass transfer barriers induced by the top gelled ECM layer, complex molecular composition of ECM with batch-to-batch variati...
متن کاملDangling OD confined in a Langmuir monolayer.
Novel structural features of water confined in lipid Langmuir monolayers are revealed. Using vibrational sum frequency generation spectroscopy at the air/D2O/monolayer interface, dangling OD bonds were investigated. Upon increasing the monolayer surface coverage, the dangling OD stretching mode showed a marked frequency red-shift as well as spectral structure. Furthermore, the dangling OD was f...
متن کاملHuman disc cells in monolayer vs 3D culture: cell shape, division and matrix formation
BACKGROUND The relationship between cell shape, proliferation, and extracellular matrix (ECM) production, important aspects of cell behavior, is examined in a little-studied cell type, the human annulus cell from the intervertebral disc, during monolayer vs three-dimensional (3D) culture. RESULTS Three experimental studies showed that cells respond specifically to culture microenvironments by...
متن کاملElectromelting of confined monolayer ice.
In sharp contrast to the prevailing view that electric fields promote water freezing, here we show by molecular dynamics simulations that monolayer ice confined between two parallel plates can melt into liquid water under a perpendicularly applied electric field. The melting temperature of the monolayer ice decreases with the increasing strength of the external field due to the field-induced di...
متن کاملComparison of radiosensitizing effect of Resveratrol on monolayer and spheroid culture of DU145 prostatic cell line
Background: Radiotherapy is an established therapeutic modality for prostate cancer. Resveratrol, a natural antioxidant, has been shown to inhibit carcinogenesis and to block the process of tumor initiation and progression. No data is available on the response of cellular spheroid to Reseveratol. In this study we have examined the effect of Resveratol on the radiation response of human p...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Cytometry Part A
سال: 2006
ISSN: 1552-4922,1552-4930
DOI: 10.1002/cyto.a.20341